| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Lactococcus lactis | AGGCGTTCCAATGGATGCT | TGCAGCTCTCTCACGCAA | 59.70 | 59.26 | 163 | 117 |
100.00%
|
75.00%
|
| Primer 32 | Lactococcus lactis | AGGCGTTCCAATGGATGCT | TTGCAGCTCTCTCACGCA | 59.70 | 59.26 | 164 | 117 |
100.00%
|
75.00%
|
| Primer 33 | Lactococcus lactis | AGGCGTTCCAATGGATGCT | TCACGCAAAGCTCCCTCTG | 59.70 | 60.00 | 153 | 117 |
100.00%
|
75.00%
|
| Primer 34 | Lactococcus lactis | AGGCGTTCCAATGGATGCT | ACGCAAAGCTCCCTCTGT | 59.70 | 58.85 | 151 | 117 |
100.00%
|
75.00%
|
| Primer 35 | Lactococcus lactis | AGGCGTTCCAATGGATGCT | TCTCACGCAAAGCTCCCTC | 59.70 | 59.71 | 155 | 117 |
100.00%
|
75.00%
|
| Primer 36 | Lactococcus lactis | ACTGGTGGGTCAGCAAGTG | TCAACCGCTCGTCCATACTG | 59.85 | 59.83 | 221 | 117 |
100.00%
|
75.00%
|
| Primer 37 | Lactococcus lactis | GTTACGGCTACGCTCGTGT | TCGGCTTTGGTCGCAACT | 60.15 | 59.89 | 202 | 116 |
100.00%
|
75.00%
|
| Primer 38 | Lactococcus lactis | TTACGGCTACGCTCGTGT | TCGGCTTTGGTCGCAACT | 58.73 | 59.89 | 201 | 116 |
100.00%
|
75.00%
|
| Primer 39 | Lactococcus lactis | TGACGTACCTGGTTACGGC | TCGGCTTTGGTCGCAACT | 59.41 | 59.89 | 213 | 116 |
100.00%
|
75.00%
|
| Primer 40 | Lactococcus lactis | GCGGAAATTTGCTTGGGC | AGCCCAAACGCGTTGACT | 58.13 | 60.20 | 275 | 116 |
100.00%
|
75.00%
|